Biology - Biotechnology - Recombinant DNA technology - Making Recombinant DNA
6-step process for producing recombinant insulin. Step 1: 'IDENTIFY the gene of interest. Find and isolate the human insulin gene.' Step 2: 'CUT with RESTRICTION ENZYMES. These molecular scissors cut DNA at specific palindromic sequences (e.g., GAATTC). They leave 'sticky ends' - single-stranded ove